Learn about Kinetic Exclusion Assays and how they are used to measure affinity and kinetics.
Interactive Theory Curve
An interactive theoretical binding curve for both equilibrium and kinetic curves.
Advantages of a Kinetic Exclusion Assay (KinExA)
- Can measure cellularly expressed proteins.
- Measurements can be made with unpurified materials. (e.g. serum, cell lysates, ascites fluid, supernatants, etc.)
- Measures a wide range of binding constants.
- Versatile assay formats.
- Can identify non-standard binding interactions such as cooperativity and mixtures.
- No risk of mass transport limitations or artifacts that may mask true binding constants.
- Highly reproducible.
The KinExA 3100 uses the kinetic exclusion assay platform to provide a premium method to measure binding. The superior sensitivity of the instrument is due to a combination of patented flow cell technology, precision fluidics, and specialized optics.
The KinExA 3200 is an improved model also employing the kinetic exclusion assay platform. Enhanced modifications offer additional experiment validation and efficiency, thereby increasing productivity.
The Autosampler provides KinExA 3200 users increased productivity by enabling unattended operation though multiple experiments. It is a versatile and efficient addition to assist in the determination of binding characteristics.